UVP Releases White Paper on Multiple Color Bacteria Counting Technique

UVP, LLC offers a white paper defining the technique to count multiple color bacteria (Focal Points FP-149). The ColonyDoc-It Imaging Station captures brilliant color colony images. The software then differentiates between multiple colors when counting the colonies. The white paper describes the materials and methods used for differentiating between b-glucuronidase positive (blue) bacteria and negative (yellow) bacteria.

The results generated shows detailed counts of each color and percentage of the population determined to be positive in b-glucuronidase. Janelle Batman, UVP Product Manager states, “the ColonyDoc-It’s high resolution color camera and advanced software algorithms provide researchers the means to finely differentiate among the myriad colors expressed by colonies.“

The ColonyDoc-It Station’s innovative design enables users to automatically count colonies quickly and accurately. The station features:

• Integrated high resolution digital color camera for brilliant color colony images
• Several light sources for illuminating a wide range of stained media
• Use of pour, spread and spiral plates and filters with sizes from 33 to 150mm
• Capture colony sizes as small as 0.08mm
• Count colonies automatically and generate detailed statistics
• Doors create a darkroom environment
• GFP fluorescence with epi blue light and optional GFP filter

The ColonyDoc-It Station allows time lapse counting and applications such as microbiology, quality control, environmental and pharmaceutical applications.