HORIBA Jobin Yvon Offers Fluorescence Lifetimes on a Microscope

Now you can add a new dimension in molecular dynamics to your microscope with Time-Correlated Single-Photon Counting (TCSPC).

Time-resolved microscopy is the ultimate tool for investigating any dynamic events in cells and subcellular structures, such as energy-transfer and molecular binding. HORIBA Jobin Yvon, the leader in fluorescence spectroscopy, offers an advanced system to apply time-resolved fluorescence spectroscopy on the micron-scale. We have adapted IBH TCSPC components for use with a microscope to achieve the best sensitivity for fluorescence-lifetime measurement. Light sources are interchangeable pulsed-diode sources, including the first deep-UV diodes: 280 nm, 295 nm and 340 nm LEDs. With the 280 nm LED, you can measure fluorescence lifetimes of proteins with tryptophan directly from tissue samples. The system can measure lifetimes from 100 ps up to 100 µs with TCSPC, and phosphorescence lifetimes (>10 µs) with MCS using the state-of-the-art IBH TBX-04 PMT detector and electronics.