Olympus has released the NA 1.7 APON100×HOTIRF objective for imaging intracellular molecules. The objective features a numerical aperture (NA) of 1.7 that helps attain the maximum Z-resolution for a Total Internal Reflection Fluorescence (TIRF) microscope.
New Olympus NA 1.7 APON100×HOTIRF objective lens
TIRF microscopy ensures high signal-to-noise ratio and very thin penetration depth. Its lens can be efficiently used for super resolution applications and also for performing single molecule dynamic studies.
Olympus’ NA 1.7 APON100×HOTIRF objective is suitable for a range of applications that entail sensitive fluorescent detection and super-high Z-resolution. Its unique optical design combined with a special sapphire glass integrated with low auto-fluorescence immersion oil having maximum refraction index enables achieving a variety of imaging applications. The lens reduces the background emission from molecules present in the surrounding solution.
The newly designed TIRF objective ensures maximum optical performance needed for carrying out TIRF co-localisation studies and super resolution applications. The thin optical slices present at the specimen’s surface generate enhanced spatial resolution. The new TIRF objective features x 100 magnification, which is suitable for in vitro TIRF microscopy for observing cytoskeletal dynamics or any other cellular components in solution.
Olympus is a well-established company that offers advanced TIRF microscopy solutions including the first commercial TIRF system. With expertise in technical innovation, Olympus has delivered the largest range of TIRF objectives and the cell^tirf illuminator that supports molecular interactions studies. The illuminator features exceptional independent laser control, advanced optics, and unique precision. The ultra-sensitive simultaneous multi-colour TIRF microscopy includes independent beam paths and 4 laser channels. Being incorporated with Olympus xcellence system ensures achieving versatile, rapid visualization of intracellular molecular events with enhanced resolution.